normal human osteoblast cells nhost locc2538  (Lonza)

Journal: Materials

Article Title: Conductive Polyaniline Patterns on Electrospun Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering

doi: 10.3390/ma14174837

Figure Lengend Snippet: Morphology of NHOst cel culture for ( a – d ) 3 and ( e , f ) 7 days on the PCL/n-HAp electrospun materials ( a , b , e , f ) and PCL/n-HAp/PANI materials ( c , d , g , h ). Scale bar is 200 μm. Red arrows indicate areas with polyaniline applied.

Article Snippet: Normal human osteoblast cells (NHOst) (Lonza, Tampa, FL, USA) were used and cultured on obtained scaffolds.

Techniques:

Journal: Materials

Article Title: Conductive Polyaniline Patterns on Electrospun Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering

doi: 10.3390/ma14174837

Figure Lengend Snippet: ( a ) Alkaline phosphatase activity test; ( b ) Mineralization progress measured by concentration of hydroxyapatites of NHOst cell culture on obtained materials. *: the results are statistically significant.

Article Snippet: Normal human osteoblast cells (NHOst) (Lonza, Tampa, FL, USA) were used and cultured on obtained scaffolds.

Techniques: Activity Assay, Concentration Assay, Cell Culture

Journal: Materials

Article Title: Conductive Polyaniline Patterns on Electrospun Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering

doi: 10.3390/ma14174837

Figure Lengend Snippet: Scanning electron microscopy images of normal human osteoblast (NHOst) cells adhered to PCL/n-HAp ( a – d ) and PCL/n-HAp/PANI scaffold after 7 days of cell culture. The edges of growing cells are marked with arrows ( e – h ).

Article Snippet: Normal human osteoblast cells (NHOst) (Lonza, Tampa, FL, USA) were used and cultured on obtained scaffolds.

Techniques: Electron Microscopy, Cell Culture

Journal: International Journal of Spine Surgery

Article Title: Cell and Tissue Response to Polyethylene Terephthalate Mesh Containing Bone Allograft in Vitro and in Vivo

doi: 10.14444/7135

Figure Lengend Snippet: Design of normal human osteoblast experiment.

Article Snippet: In Vitro Culture Human MSCs (male donor) and normal human osteoblasts (NHOst cells, male donor), as well as MSC growth media (MSCGM) and osteogenic media (OM), were purchased from Lonza (Basel, Switzerland).

Techniques:

Journal: International Journal of Spine Surgery

Article Title: Cell and Tissue Response to Polyethylene Terephthalate Mesh Containing Bone Allograft in Vitro and in Vivo

doi: 10.14444/7135

Figure Lengend Snippet: In vitro normal human osteoblasts (NHOst) response of cells cultured on tissue culture plastic (TCPS) or particulate bone graft (mineralized bone matrix [MBM]) in the presence or absence of polyethylene terephthalate mesh (PET). Response was examined by measuring markers that indicate the initiation of differentiation including (A) DNA; (B) alkaline phosphate specific activity; factors that indicate late stage osteoblast differentiation including (C) osteocalcin and (D) osteoprotegerin (OPG); and production of factors associated with osteogenesis, including bone morphogenetic proteins (BMPs) 2 (E) and 4 (F) and (G) vascular endothelial growth factor-A (VEGF). P < .05 # versus no mesh, @ versus no MBM.

Article Snippet: In Vitro Culture Human MSCs (male donor) and normal human osteoblasts (NHOst cells, male donor), as well as MSC growth media (MSCGM) and osteogenic media (OM), were purchased from Lonza (Basel, Switzerland).

Techniques: In Vitro, Cell Culture, Activity Assay

Journal: International Journal of Spine Surgery

Article Title: Cell and Tissue Response to Polyethylene Terephthalate Mesh Containing Bone Allograft in Vitro and in Vivo

doi: 10.14444/7135

Figure Lengend Snippet: In vitro bone marrow stromal cell (MSC) response of cells cultured on tissue culture plastic (TCPS) or mineralized bone matrix (MBM), with MSC growth media (GM) or osteogenic media (OM), in the presence or absence of polyethylene terephthalate mesh (PET). Response was examined by measuring markers that indicate the initiation of differentiation including (A) DNA; (B) alkaline phosphate specific activity; factors that indicate late stage osteoblast differentiation including (C) osteocalcin and (D) osteoprotegerin (OPG); and production of factors associated with osteogenesis, including bone morphogenetic proteins (BMPs) 2 (E) and 4 (F) and (G) vascular endothelial growth factor-A (VEGF). P < .05 # versus no mesh, @ versus no MBM. % versus GM.

Article Snippet: In Vitro Culture Human MSCs (male donor) and normal human osteoblasts (NHOst cells, male donor), as well as MSC growth media (MSCGM) and osteogenic media (OM), were purchased from Lonza (Basel, Switzerland).

Techniques: In Vitro, Cell Culture, Activity Assay